LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1922..2018) /label=attL2 /note="attL2 (c)" misc_signal complement(2033..2052) /note="T7 Promoter/priming site (c)" primer_bind 2060..2076 /note="M13 Reverse priming site" gene 2189..2998 /note="Kanamycin resistance gene" rep_origin 3119..3792 /note="pUC origin" vector join(1930..3795,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1915 /dnas_title="CHST9-Nterm-TEVfusion-pDONR221" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="18" /map="18q12" CDS 692..1915 /dnas_title="N-acetylgalactosamine 4-O-sulfotransferase 2 GalNAc4ST-2" /note="GalNAc-4-O-sulfotransferase 2" /codon_start=1 /product="N-acetylgalactosamine 4-O-sulfotransferase 2 GalNAc4ST-2" /protein_id="AAK01862.1" /db_xref="GI:12711481" /translation="MVMNPKQVFLSVLIFGVAGLLLFMYLQVWIEEQHTGRVEKRREQ KVTSGWGPVKYLRPVPRIMSTEKIQEHITNQNPKFHMPEDVREKKENLLLNSERSTRL LTKTSHSQGGDQALSKSTGSPTEKLIEKHQGAKTVFNKFSNMDWPVDIHPLNKSLVKD NKWKKTEETQEKRRSFLQEFCKKYGGVSHHQSHLFHTVSRIYVEDKHKILYCEVPKAG CSNWKRILMVLNGLASSAYNISHNAVHYGKHLKKLDSFDLKGIYTRLNTYTKAVFVRD PMERLVSAFRDKFEHPNSYYHPVFGKAIIKKYRPNACEEALINGSGVKFKEFIHYLLD SHRPVGMDIHWEKVSKLCYPCLINYDFVGKFETLEEDANYFLQMIGAPKELKFPNFKD RHSSDERTNAQVVRQYLKDLTRTERQLIYDFYYLDYLMFNYTTPFL" misc_feature 692..1915 /note="CHST9 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgaagaacaa catacaggga gagtggagaa 721 gagaagagaa caaaaagtaa cttcaggatg gggaccagtg aagtacttgc ggcctgtacc 781 cagaatcatg agtacagaaa aaatccagga acatatcacc aaccagaacc ccaagtttca 841 catgcctgag gatgtacgag aaaaaaagga aaatcttcta ctcaattctg agagatctac 901 taggctctta acaaagacca gtcattcaca aggaggggat caagctttaa gtaagtccac 961 agggtcacca acagagaagt tgattgaaaa acatcaagga gctaagactg tttttaacaa 1021 gttcagcaac atggattggc cagtggacat tcacccttta aacaaaagtt tagtcaaaga 1081 taataaatgg aagaaaactg aggagaccca agagaaacga aggtctttcc ttcaggagtt 1141 ttgcaagaaa tacggtgggg tgagtcatca tcagtcacat ctttttcata cagtatccag 1201 aatctatgta gaagataaac acaaaatctt atattgtgag gtacctaagg ctggctgttc 1261 caattggaaa agaattctga tggtactaaa tggattggct tcctctgcat acaacatctc 1321 ccacaatgct gtccactacg ggaagcattt gaagaagcta gatagctttg acctaaaagg 1381 gatatatacc cgcttaaata cttacaccaa agctgtgttt gttcgtgatc ccatggaaag 1441 attagtatca gcctttaggg acaaatttga acaccccaat agttattacc atccagtatt 1501 cggaaaggca attatcaaga aatatcgacc aaatgcctgt gaagaagcat taattaatgg 1561 atctggagtc aagttcaaag agtttatcca ctacttgctg gattcccacc gtccagtagg 1621 aatggacatt cactgggaaa aggtcagcaa actctgctat ccgtgtttga tcaactatga 1681 ttttgtaggg aaatttgaga ctttggaaga agatgccaat tactttttac agatgatcgg 1741 tgctccaaag gagctgaaat ttcccaactt taaggatagg cactcttccg atgaaagaac 1801 caatgctcaa gtcgtgagac agtatttaaa ggatctgact agaactgaga gacaattaat 1861 ctatgacttt tattacttgg actatttaat gtttaattat acaactccat ttttgTAGGA 1921 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 1981 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2041 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2101 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2161 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2221 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2281 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2341 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2401 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2461 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2521 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2581 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2641 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2701 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2761 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2821 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2881 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 2941 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3001 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3061 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3121 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3181 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3241 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3301 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3361 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3421 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3481 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3541 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3601 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3661 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3721 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3781 TTTTGCTCAC ATGTT //